Science: Atmospheric pressure MALDI.
نویسندگان
چکیده
منابع مشابه
Atmospheric pressure MALDI/ion trap mass spectrometry.
A new sample ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was coupled with a commercial ion trap mass spectrometer. This configuration enables the application-specific selection of external atmospheric ionization sources: the electrospray/APCI (commercially available) and AP MALDI (built in-house), which can be readily interchanged within mi...
متن کاملAtmospheric pressure molecular imaging by infrared MALDI mass spectrometry.
An atmospheric pressure (AP) MALDI imaging interface was developed for an orthogonal acceleration time-of-flight mass spectrometer and utilized to analyze peptides, carbohydrates, and other small biomolecules using infrared laser excitation. In molecular imaging experiments, the spatial distribution of mock peptide patterns was recovered with a detection limit of approximately 1 fmol/pixel from...
متن کاملAtmospheric pressure infrared MALDI imaging mass spectrometry for plant metabolomics.
The utility of atmospheric pressure infrared MALDI mass spectrometry (AP IR-MALDI) was assessed for plant metabolomics studies. Tissue sections from plant organs, including flowers, ovaries, aggregate fruits, fruits, leaves, tubers, bulbs, and seeds were studied in both positive and negative ion modes. For leaves, single laser pulses sampled the cuticle and upper epidermal cells, whereas multip...
متن کاملAtmospheric pressure MALDI-FTMS of normal and chemically modified RNA.
Atmospheric pressure (AP) MALDI has been combined with Fourier transform mass spectrometry (FTMS) to obtain the unambiguous characterization of RNA samples modified by solvent accessibility reagents used in structural studies of RNA and protein-RNA complexes. The formation of cation adducts typical of MS analysis of nucleic acids was effectively reduced by extensive washing of the anionic analy...
متن کاملAtmospheric pressure MALDI Fourier transform mass spectrometry of labile oligosaccharides.
An atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) source coupled to Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) under UV laser and solid matrix conditions has been demonstrated to analyze a variety of labile oligosaccharides including O-linked and N-linked complex glycans released from glycoproteins. Spectra were acquired by both AP MALDI...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2000
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac002757e